Journal: The EMBO Journal

Article Title: Global analysis of cancer cell responses to USP9X inhibition

doi: 10.1038/s44318-026-00742-y

Figure Lengend Snippet: ( A ) Schematic of short-term cell killing and long-term clonogenic potential experiments. ( B ) NCI-60 cancer cell panel of 57 cancer cell lines treated with WEHI-092 (10 µM). Data shown are the normalised cell growth at 3 days post treatment relative to untreated control and relative to day 0 for each individual cell line. Negative values indicate cell death at 3 days post treatment. Cell lines are colour-coded depending on their cancer type. NSC - non-small cell. CNS - central nervous system. The data shown are the mean of two technical replicates. ( C ) IncuCyte live-cell imaging cell death data for cell lines treated with WEHI-092, used at 10 µM for UO-31, A498, ACHN, CAKI-1, HMEC-1, HDF cells and at 17 µM for the remaining cell lines. Cell death was measured by PI uptake. The data shown are the mean of at least two independent biological repeats with two technical replicates per experiment. FOC fold-over control, HDF human dermal fibroblasts. Error bars, s.e.m. ( D ) IncuCyte live-cell imaging proliferation data for cell lines treated with WEHI-092. Data shown is the mean of at least two independent biological repeats with two technical replicates per experiment at 72 h post treatment where each experiment is shown as a single datapoint. Data were normalised to the untreated condition. ( E ) Clonogenic potential assay for cell lines treated with WEHI-092. Representative images from three independent biological repeats. ( F ) Quantification of clonogenic potential assays, as shown in ( E ). Data shown are the mean of three independent biological repeats relative to the untreated condition (dashed line) for each cell line, where each separate experiment is shown as a single datapoint. Bars are colour-coded as in ( B ). .

Article Snippet: IncuCyte software , Intellicyt , v2021A_20211.2.0.0.

Techniques: Control, Live Cell Imaging